|
KMID : 0894520100140040253
|
|
Development & Reproduction
2010 Volume.14 No. 4 p.253 ~ p.259
|
|
|
Methylation Patterns of Imprinting Genes, H19, Igf2r, and Snrpn, in Mouse Embryonic Stem Cells and Nuclear Transferred Embryonic Stem Cells
|
|
Lee Min-Ho
Ju Jin-Young
Cho Youl-Hee
Shim Sung-Han
|
|
|
Abstract
|
|
|
|
DNA methylation is one of the major epigenetic regulations of gene expression. The DNA methylation patterns are dramatically changed during gametogenesis and embryogenesis, and especially, it has been known that embryonic stem cells show a distinct methylation pattern. In this study, we examined the methylation patterns of imprinting genes, H19, Igf2r, and Snrpn, in stem cells induced from fertilized embryo (fES) and somatic cell nuclear transferred embryo (ntES). The methylation pattern of H19 gene in both fES and ntES were similar. However, the methylation patterns of Igf2r and Snrpn in ntES (hypermethylated) were slightly different from fES cells.
|
|
|
KEYWORD
|
|
|
Imprinting genes, Methylation pattern, Embryonic stem cells
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|